Revalorización de subproductos cítricos. Determinación de flavonoides por cromatográfia líquida
DOI:
https://doi.org/10.64876/radi.v28.4Palabras clave:
Cromatografía líquida, flavanonas, hesperidina, residuos cítricosResumen
Se desarrolló una metodología analítica para la determinación de flavonoides en extractos de cáscaras de cítricos, mediante cromatografía líquida de alta resolución acoplada a un detector de longitud de onda múltiple. Para superar las limitaciones del detector, se implementó la estrategia de reconstrucción de espectros de absorción, la cual dotó al sistema de capacidades similares a las de un detector de arreglo de diodos. Se determinó el solvente más adecuado para la separación cromatográfica y, para resolver la coelución derivada de la complejidad química de las matrices, se procedió al reajuste del gradiente de elución hasta obtener una resolución adecuada. La aplicación de la metodología permitió la identificación de hesperidina, naringina y neohesperidina, así como la categorización de picos no identificados dentro de la familia de las flavanonas. En conclusión, se obtuvo una herramienta analítica robusta y accesible, orientada a la revalorización de residuos citrícolas como fuente de compuestos bioactivos.
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